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h9 human embryonic stem cells hescs  (WiCell Research Institute Inc)


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    WiCell Research Institute Inc h9 human embryonic stem cells hescs
    Culturing human embryonic stem cells in ColTubes. (A) Phase-contrast images of <t>H9</t> <t>hESCs</t> cultured in ColTubes at days 0, 1, 3, 5, and 7. (B) Live/Dead staining of H9 cells inside the tubes and released from the tubes on day 7. (C) Flow cytometry analysis shows that 99.6% of cells harvested on day 7 are Calcein AM-positive (live cells). (D) Flow cytometry analysis confirms that majority cells on day 7 express pluripotency markers OCT4 and Nanog.
    H9 Human Embryonic Stem Cells Hescs, supplied by WiCell Research Institute Inc, used in various techniques. Bioz Stars score: 99/100, based on 3773 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/h9 human embryonic stem cells hescs/product/WiCell Research Institute Inc
    Average 99 stars, based on 3773 article reviews
    h9 human embryonic stem cells hescs - by Bioz Stars, 2026-03
    99/100 stars

    Images

    1) Product Images from "Collagen hydrogel tube microbioreactors for cell and tissue manufacturing"

    Article Title: Collagen hydrogel tube microbioreactors for cell and tissue manufacturing

    Journal: Biofabrication

    doi: 10.1088/1758-5090/ae2718

    Culturing human embryonic stem cells in ColTubes. (A) Phase-contrast images of H9 hESCs cultured in ColTubes at days 0, 1, 3, 5, and 7. (B) Live/Dead staining of H9 cells inside the tubes and released from the tubes on day 7. (C) Flow cytometry analysis shows that 99.6% of cells harvested on day 7 are Calcein AM-positive (live cells). (D) Flow cytometry analysis confirms that majority cells on day 7 express pluripotency markers OCT4 and Nanog.
    Figure Legend Snippet: Culturing human embryonic stem cells in ColTubes. (A) Phase-contrast images of H9 hESCs cultured in ColTubes at days 0, 1, 3, 5, and 7. (B) Live/Dead staining of H9 cells inside the tubes and released from the tubes on day 7. (C) Flow cytometry analysis shows that 99.6% of cells harvested on day 7 are Calcein AM-positive (live cells). (D) Flow cytometry analysis confirms that majority cells on day 7 express pluripotency markers OCT4 and Nanog.

    Techniques Used: Cell Culture, Staining, Flow Cytometry

    Differentiating H9 hESCs into Cardiomyocytes in ColTubes. (A) The cardiomyocyte production protocol. H9 hESCs are processed into ColTubes and expanded in E8 medium, followed by mesoderm induction for 1 d, cardiomyocyte differentiation from days 2–11, and metabolic enrichment from days 11–18. (B) Phase-contrast and fluorescent images of cells in ColTubes on days 0, 1, 3, 5, 7, 11, 15, and 18. Cardiomyocytes are cTnT-positive.
    Figure Legend Snippet: Differentiating H9 hESCs into Cardiomyocytes in ColTubes. (A) The cardiomyocyte production protocol. H9 hESCs are processed into ColTubes and expanded in E8 medium, followed by mesoderm induction for 1 d, cardiomyocyte differentiation from days 2–11, and metabolic enrichment from days 11–18. (B) Phase-contrast and fluorescent images of cells in ColTubes on days 0, 1, 3, 5, 7, 11, 15, and 18. Cardiomyocytes are cTnT-positive.

    Techniques Used:



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    Image Search Results


    Culturing human embryonic stem cells in ColTubes. (A) Phase-contrast images of H9 hESCs cultured in ColTubes at days 0, 1, 3, 5, and 7. (B) Live/Dead staining of H9 cells inside the tubes and released from the tubes on day 7. (C) Flow cytometry analysis shows that 99.6% of cells harvested on day 7 are Calcein AM-positive (live cells). (D) Flow cytometry analysis confirms that majority cells on day 7 express pluripotency markers OCT4 and Nanog.

    Journal: Biofabrication

    Article Title: Collagen hydrogel tube microbioreactors for cell and tissue manufacturing

    doi: 10.1088/1758-5090/ae2718

    Figure Lengend Snippet: Culturing human embryonic stem cells in ColTubes. (A) Phase-contrast images of H9 hESCs cultured in ColTubes at days 0, 1, 3, 5, and 7. (B) Live/Dead staining of H9 cells inside the tubes and released from the tubes on day 7. (C) Flow cytometry analysis shows that 99.6% of cells harvested on day 7 are Calcein AM-positive (live cells). (D) Flow cytometry analysis confirms that majority cells on day 7 express pluripotency markers OCT4 and Nanog.

    Article Snippet: For a typical cell culture, H9 human embryonic stem cells (hESCs) (WA09, WiCell) loaded in 20 μ l ColTubes were suspended in 2 ml Essential 8 medium (Gibco) supplemented with 10 μ M Y-27632 (Sigma) in a 6-well plate and incubated at 37 °C with 5% CO2 and 21% O2.

    Techniques: Cell Culture, Staining, Flow Cytometry

    Differentiating H9 hESCs into Cardiomyocytes in ColTubes. (A) The cardiomyocyte production protocol. H9 hESCs are processed into ColTubes and expanded in E8 medium, followed by mesoderm induction for 1 d, cardiomyocyte differentiation from days 2–11, and metabolic enrichment from days 11–18. (B) Phase-contrast and fluorescent images of cells in ColTubes on days 0, 1, 3, 5, 7, 11, 15, and 18. Cardiomyocytes are cTnT-positive.

    Journal: Biofabrication

    Article Title: Collagen hydrogel tube microbioreactors for cell and tissue manufacturing

    doi: 10.1088/1758-5090/ae2718

    Figure Lengend Snippet: Differentiating H9 hESCs into Cardiomyocytes in ColTubes. (A) The cardiomyocyte production protocol. H9 hESCs are processed into ColTubes and expanded in E8 medium, followed by mesoderm induction for 1 d, cardiomyocyte differentiation from days 2–11, and metabolic enrichment from days 11–18. (B) Phase-contrast and fluorescent images of cells in ColTubes on days 0, 1, 3, 5, 7, 11, 15, and 18. Cardiomyocytes are cTnT-positive.

    Article Snippet: For a typical cell culture, H9 human embryonic stem cells (hESCs) (WA09, WiCell) loaded in 20 μ l ColTubes were suspended in 2 ml Essential 8 medium (Gibco) supplemented with 10 μ M Y-27632 (Sigma) in a 6-well plate and incubated at 37 °C with 5% CO2 and 21% O2.

    Techniques: